MICROPROPAGATION OF CALOPHACA WOLGARICA

Abstract

Calophaca wolgarica (L. fil.) Fisch. ex DC. is an endangered plant included into Russian Federation Red Book. The central purpose of the experiment is to establish an efficient micropropagation method for rapid multiplication of Calophaca planting material in order to solve problems related to repatriation and introduction of this plant. The best method of micropropagation of this shrub is micrograftage by removing the apical dominance. An optimal sterilization method of seed explants is found. It is shown that the most effective way to decontaminate seeds of Calophaca wolgarica is firing in the burner flame. The optimum concentration of phytohormones (2 mg.l
BA and 0,1 mg.l
NAA) on MS medium was found to obtain the greatest number of mericlones. The best conditions for rooting were 1/2MS medium supplemented with 0,5 mg.l
IBA.